Mineral particles modulate osteo-chondrogenic differentiation of embryonic stem cell aggregates.

摘要: Abstract Pluripotent stem cell aggregates offer an attractive approach to emulate embryonic morphogenesis and skeletal development. Calcium phosphate (CaP) based biomaterials have been shown promote bone healing due their osteoconductive potential osteoinductive properties. In this study, we hypothesized that incorporation of CaP-coated hydroxyapatite mineral particles (MPs) within murine (ESC) could osteo-chondrogenic differentiation. Our results demonstrated MP alone dose-dependently promoted the gene expression chondrogenic early osteogenic markers. combination with soluble cues, MPs enhanced hypertrophic phenotype, mineralization ESC aggregates. Additionally, reduced pluripotency thereby decreased size teratomas derived from MP-incorporated in vivo . data suggested a novel yet simple means using control fate create osteochondral niche for tissue engineering applications. Statement Significance Directing differentiation via represents strategy fates formation. study demonstrates ability calcium phosphate-based osteochondrogenic as well modulate teratoma formation This hybrid biomaterial–ESC aggregate serves enabling platform evaluate regulate regenerate functional tissues clinical