Bone regeneration in critically sized rat mandible defects through the endochondral pathway using hydroxyapatite-coated 3D-printed Ti6Al4V scaffolds

摘要: The endochondral approach has been proved to be a promising pathway in bone tissue engineering. However, whether it is suitable for repairing critically sized mandible defects unknown. We designed Ti6Al4V scaffolds with shape and pore size by 3D-printing selective-laser-melting technique implement this approach. In order improve the surface bioactivity of scaffolds, hydroxyapatite (HA) coatings (HA/L group HA/H group) different crystallite were prepared on via electrochemical deposition. Rat mesenchymal stem cells (BMSCs) seeded onto chondrogenically differentiated vitro 4 weeks then implanted into rat 8 weeks. bare scaffold HA characterized field emission scanning electron microscopy, water contact angle measurements X-ray diffractometry. Cell proliferation results showed that could better growth rate BMSCs compared surface. Additionally, safranin O staining abundant cartilage matrix chondrocytes coated scaffold. Analyses using qPCR detected higher expression chondrogenic-related gene Col2α1 vegfα groups, especially group. Together these data demonstrate coating chondrogenic differentiation BMSCs. vivo, methylene blue histological sections micro-computed tomography revealed HA-coated group, increased new formation ossification control Therefore, strategy provides an alternative method larger crystals was superior those smaller crystals.