Low-Affinity Neurotrophin Receptor p75 Promotes the Transduction of Targeted Lentiviral Vectors to Cholinergic Neurons of Rat Basal Forebrain.

摘要: Basal forebrain cholinergic neurons (BFCNs) are one of the most affected neuronal types in Alzheimer’s disease (AD), with their extensive loss documented at late stages pathology. While discriminatory provision neuroprotective agents and trophic factors to these cells is thought be substantial therapeutic potential, intricate topography structure system imposes a major challenge. To overcome this, we took advantage physiological enrichment BFCNs low-affinity p75 neurotrophin receptor (p75NTR) for targeting by lentiviral vectors within intact brain adult rat. Herein, method described that affords selective effective transduction green fluorescence protein (GFP) reporter, which combines streptavidin–biotin technology anti-p75NTR antibody-coated vectors. Specific GFP expression was attained medial septum nuclei diagonal band Broca after single intraventricular administration such targeted Bioelectrical activity GFP-labeled proven unchanged. Thus, proof principle obtained utility p75NTR vivo.