Evaluation of human immunodeficiency virus seroprevalence in population surveys using pooled sera.

摘要: The pooling of individual serum samples to determine human immunodeficiency virus (HIV) seropositivity was examined assess whether testing pooled sera technically feasible, cost-effective, and accurate for estimating seroprevalence in large population surveys. sensitivities specificities three commercially available HIV enzyme-linked immunosorbent assay (ELISA) kits were tested using 65 pools 15 each (975 total samples) at two different dilutions. With sera, the Organon Teknika Bio-EnzaBead ELISA half dilution recommended by manufacturer showed best agreement with Western blot results sera. In subsequently 92 pools, containing from a American patients attending sexually transmitted diseases clinic, estimated 5.27 compared 4.93% test 1,380 5.19% 4,028 same population. an evaluation African 10 per pool, 5.79 6.16% These indicate that is highly sensitive specific appears be cost-effective means population-based