Anti-inflammatory therapies in TRAMP mice: delay in PCa progression
作者： Larissa Akemi Kido , Fabio Montico , Rafael Sauce , Aline Barbosa Macedo , Elaine Minatel
DOI: 10.1530/ERC-15-0540
关键词: Tramp 、 Prostate 、 Cancer 、 Intraepithelial neoplasia 、 Immunohistochemistry 、 Adenocarcinoma 、 Celecoxib 、 Cancer research 、 Prostate cancer 、 Medicine
摘要: The aim of this study was to characterize the structural and molecular biology as well evaluate immediate late responses prostatic cancer in transgenic adenocarcinoma mouse prostate (TRAMP) model after treatment with goniothalamin (GTN) celecoxib. treated mice received GTN (150 mg/kg, gavage) or celecoxib (10 from 8 12 weeks age. They were killed at different ages: immediate-response groups late-response 22 weeks. ventral collected for light microscopy, immunohistochemistry, western blotting, TUNEL, ELISA. Morphological analyses indicated that delayed progression adenocarcinoma, leading a significant decrease lesion frequency both experimental period treatment, mainly high-grade intraepithelial neoplasia well-differentiated adenocarcinoma. Also, showed particular proliferative processes (PCNA) periods. Despite diminishing COX2 IGFR1 levels, presented higher action spectrum considering greater number involved inflammatory cancer. Goniothalamin attenuated pro-inflammatory response TRAMP microenvironment, delaying (PCa) progression. Celecoxib efficient regulation mice, advanced disease grade. Finally, we concluded process control early grades PCa crucial downregulation signaling pathways grades.
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