Semiautomated Assessment of Loss of Heterozygosity and Replication Error in Tumors

摘要: Loss of heterozygosity (LOH) and replication error (RER) are important phenomena in tumor development, with diagnostic prognostic relevance. Therefore, screening for LOH RER is a desirable first step the molecular analysis tumors. We used semiautomated procedures based on multicolor fluorescently labeled microsatellite markers an automated sequencer PCR amplification, electrophoresis products, allele detection set 16 microsatellites 56 colorectal improved existing software computer-assisted assessment RER. A comparison these results those conventional, radioactive technique visual interpretation shows high degree correlation between two methods. The rates similar to reported previously. main advantages fluorescence-based typing objective, observer-unrelated, easy, rapid computer-based scoring, resulting quantitative