The isolation and functional characterisation of a B. napus acyl carrier protein 5' flanking region involved in the regulation of seed storage lipid synthesis.
作者： Jacqueline de Silva , Susan J. Robinson , Richard Safford
DOI: 10.1007/BF00047719
关键词: Reporter gene 、 Biochemistry 、 Biology 、 Chimeric gene 、 Nucleic acid sequence 、 5' flanking region 、 Gene 、 Gene expression 、 Regulatory sequence 、 Acyl carrier protein
摘要: Acyl carrier protein (ACP) is a key component of the fatty acid biosynthetic machinery in plants. A 1.4 kb 5' flanking region Brassica napus ACP gene (ACP05) was transcriptionally fused to reporter beta-glucuronidase (GUS), and expression chimeric monitored transgenic tobacco. GUS activity found increase through seed development reaching maximum value, coincident with most active phase storage lipid synthesis that was, on average, 100-fold higher than observed leaf. In control plants transformed CaMV 35S-GUS constructs, similar leaf all stages development. Based average values, level obtained via promoter comparable from 35S promoter. We therefore conclude isolated sequence represents strong element involved developmental regulation B. tissue. Putative regulatory elements upstream ACP05 were identified by dot matrix analysis comparison regions second seed-expressed rape an Arabidopsis gene.
springer.com
sci-hub.se 参考文章(23)
P.K. Stumpf, Chapter 6 Fatty acid biosynthesis in higher plants New Comprehensive Biochemistry. ,vol. 7, pp. 155- 179 ,(1984) , 10.1016/S0167-7306(08)60124-6
J J Doyle, M A Schuler, W D Godette, V Zenger, R N Beachy, J L Slightom, The glycosylated seed storage proteins of Glycine max and Phaseolus vulgaris. Structural homologies of genes and proteins. Journal of Biological Chemistry. ,vol. 261, pp. 9228- 9238 ,(1986) , 10.1016/S0021-9258(18)67644-6
Donald Grierson, Simon N. Covey, Structure and Expression of Nuclear Genes Springer, Dordrecht. pp. 22- 46 ,(1988) , 10.1007/978-94-010-9649-2_2
C.P. Joshi, An inspection of the domain between putative TATA box and translation start site in 79 plant genes Nucleic Acids Research. ,vol. 15, pp. 6643- 6653 ,(1987) , 10.1093/NAR/15.16.6643
A. Hoekema, P. R. Hirsch, P. J. J. Hooykaas, R. A. Schilperoort, A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid Nature. ,vol. 303, pp. 179- 180 ,(1983) , 10.1038/303179A0
M. Guiltinan, W. Marcotte, R. Quatrano, A plant leucine zipper protein that recognizes an abscisic acid response element Science. ,vol. 250, pp. 267- 271 ,(1990) , 10.1126/SCIENCE.2145628
R D Allen, F Bernier, P A Lessard, R N Beachy, Nuclear factors interact with a soybean beta-conglycinin enhancer. The Plant Cell. ,vol. 1, pp. 623- 631 ,(1989) , 10.1105/TPC.1.6.623
Anil Shirsat, Neville Wilford, Ronald Croy, Donald Boulter, Sequences responsible for the tissue specific promoter activity of a pea legumin gene in tobacco. Molecular Genetics and Genomics. ,vol. 215, pp. 326- 331 ,(1989) , 10.1007/BF00339737
Stephen L. Dellaporta, Jonathan Wood, James B. Hicks, A plant DNA minipreparation: Version II Plant Molecular Biology Reporter. ,vol. 1, pp. 19- 21 ,(1983) , 10.1007/BF02712670
Z. L. Chen, M. A. Schuler, R. N. Beachy, Functional analysis of regulatory elements in a plant embryo-specific gene Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 8560- 8564 ,(1986) , 10.1073/PNAS.83.22.8560