Isolation and characterization of an Arabidopsis biotin carboxylase gene and its promoter.
作者： Xiaoming Bao , Basil S. Shorrosh , John B. Ohlrogge
关键词: Intron 、 Biotin carboxylase 、 Molecular biology 、 Sequence analysis 、 Gene 、 Biology 、 Genetics 、 Arabidopsis 、 Acetyl-CoA carboxylase 、 Gene expression 、 Regulation of gene expression
摘要: In the plastids of most plants, acetyl-CoA carboxylase (ACCase; EC 6.4.1.2) is a multisubunit complex consisting biotin (BC), biotin-carboxyl carrier protien (BCCP), and carboxytransferase (alpha-CT, beta-CT) subunits. To better understand regulation this enzyme, we have isolated sequenced BC genomic clone from Arabidopsis partially characterized its promoter. Fifteen introns were identified. The deduced amino acid sequence mature protein highly conserved between tobacco (92.6% identity). expression was evaluated using northern blots BC/GUS fusion constructs in transgenic Arabidopsis. GUS activity transgenics as well transcript level native gene both found to be higher silique flower than root leaf. Analysis suspension cells transformed with truncated promoter/GUS fusions indicated region -140 +147 contained necessary promoter elements which supported basal expression. A positive regulatory located -2100 -140, whereas negative element possibly first intron. addition, several identified Surprisingly, although low-abundance protein, similar 35S/GUS constructs.
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sci-hub.se 参考文章(49)
Gert-Jan de Boer, Tony Fawcett, Antoni R. Slabas, H. John J. Nijkamp, Antoine R. Stuitje, Analysis of the Arabidopsis Enoyl-ACP Reductase Promoter in Transgenic Tobacco Springer Netherlands. pp. 360- 362 ,(1997) , 10.1007/978-94-017-2662-7_114
P. ROY VAGELOS, Regulation of Fatty Acid Biosynthesis Current Topics in Cellular Regulation. ,vol. 4, pp. 119- 166 ,(1971) , 10.1016/B978-0-12-152804-1.50010-6
A Weinreb, D A Collier, B K Birshtein, R D Wells, Left-handed Z-DNA and intramolecular triplex formation at the site of an unequal sister chromatid exchange Journal of Biological Chemistry. ,vol. 265, pp. 1352- 1359 ,(1990) , 10.1016/S0021-9258(19)40021-5
J J Doyle, M A Schuler, W D Godette, V Zenger, R N Beachy, J L Slightom, The glycosylated seed storage proteins of Glycine max and Phaseolus vulgaris. Structural homologies of genes and proteins. Journal of Biological Chemistry. ,vol. 261, pp. 9228- 9238 ,(1986) , 10.1016/S0021-9258(18)67644-6
Gynheung An, [17] Binary ti vectors for plant transformation and promoter analysis Methods in Enzymology. ,vol. 153, pp. 292- 305 ,(1987) , 10.1016/0076-6879(87)53060-9
D Post-Beittenmiller, J G Jaworski, J B Ohlrogge, In vivo pools of free and acylated acyl carrier proteins in spinach. Evidence for sites of regulation of fatty acid biosynthesis. Journal of Biological Chemistry. ,vol. 266, pp. 1858- 1865 ,(1991) , 10.1016/S0021-9258(18)52372-3
P.G. Roessler, J.B. Ohlrogge, Cloning and characterization of the gene that encodes acetyl-coenzyme A carboxylase in the alga Cyclotella cryptica. Journal of Biological Chemistry. ,vol. 268, pp. 19254- 19259 ,(1993) , 10.1016/S0021-9258(19)36507-X
Q Lu, L L Wallrath, H Granok, S C Elgin, CT)n (GA)n repeats and heat shock elements have distinct roles in chromatin structure and transcriptional activation of the Drosophila hsp26 gene. Molecular and Cellular Biology. ,vol. 13, pp. 2802- 2814 ,(1993) , 10.1128/MCB.13.5.2802
Robert D. Wells, David A. Collier, Jeffery C. Hanvey, Mitsuhiro Shimizu, Franz Wohlrab, The chemistry and biology of unusual DNA structures adopted by oligopurine.oligopyrimidine sequences. The FASEB Journal. ,vol. 2, pp. 2939- 2949 ,(1988) , 10.1096/FASEBJ.2.14.3053307
G. Vancanneyt, R. Schmidt, A. O'Connor-Sanchez, L. Willmitzer, M. Rocha-Sosa, Construction of an intron-containing marker gene : splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation Molecular Genetics and Genomics. ,vol. 220, pp. 245- 250 ,(1990) , 10.1007/BF00260489