Altered E-NTPDase/E-ADA activities and CD39 expression in platelets of sickle cell anemia patients.
作者： Lívia G. Castilhos , Pedro H. Doleski , Stephen A. Adefegha , Lara V. Becker , Jader B. Ruchel
DOI: 10.1016/J.BIOPHA.2016.02.009
关键词: Platelet 、 Immunology 、 Hemoglobinopathy 、 Sickle cell anemia 、 Internal medicine 、 Biology 、 Extracellular 、 Adenosine deaminase 、 Hemolysis 、 Purinergic receptor 、 Hemostasis 、 Endocrinology
摘要: Abstract Sickle cell anemia (SCA) is a hemoglobinopathy characterized by hemolysis and vaso-occlusions caused rigidly distorted red blood cells. crisis associated with extracellular release of nucleotides platelets, which are critical mediators hemostasis participating actively in purinergic thromboregulatory enzymes system.This study aimed to investigate the activities system ecto-enzymes present on platelet surface as well CD39 CD73 expressions platelets SCA treated patients. Fifteen patients 30 health subjects (control group) were selected. Ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase), ecto-5′-nucleotidase (E-5′-NT) ecto-adenosine deaminase (E-ADA) measured isolated from these individuals. Results demonstrated an increase 41 % E-NTPDase for ATP hydrolysis, 52% ADP hydrolysis 60 E-ADA activity (P
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