Membrane-binding sites for acyl carrier protein inEscherichia coli

作者: Nicolas Bayan , Helene Therisod

DOI: 10.1016/0014-5793(89)80963-9

关键词: Acyl carrier proteinMembrane proteinDissociation constantMembraneInner membraneBacterial outer membraneBinding siteBiologyBiochemistryEscherichia coliBiophysicsGeneticsCell biologyMolecular biologyStructural biology

摘要: We report that membrane vesicles of Escherichia coli contain protein-binding sites for acyl carrier protein. Scatchard analysis the binding indicates a dissociation constant around 0.35 μM and maximum number 50 pmol per mg Binding is on inner while outer devoid sites. These results are consistent with fact some protein-dependent enzymes implicated in phospholipid- membrane-derived oligosaccharide biosynthesis localized cytoplasmic membrane.

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