Turnover of fatty acids in the 1-position of phosphatidylethanolamine in Escherichia coli.

摘要: Abstract Phosphatidylethanolamine is the major membrane phospholipid of Escherichia coli, and two experimental approaches were used to investigate metabolic activity fatty acids occupying 1-position this phospholipid. [3H]Acetate pulse-chase experiments with logarithmically growing cells indicated that 3-5% acyl groups removed from phosphatidylethanolamine pool/generation. The reacylation aspect turnover cycle was demonstrated by incorporation into pre-existing when de novo biosynthesis inhibited using plsB acyltransferase mutant. 2- Acylglycerophosphoethanolamine would be intermediate in a cycle, lysophospholipid identified as component could re-esterified membrane-bound acyltransferase. either utilized acyl-acyl carrier protein directly an donor or activated for transfer presence ATP Mg2+. Acyl-acyl also latter reaction complete inhibition formation protein-specific antibodies observation LiCl reversed addition protein. Coenzyme A thioesters not substrates These results suggest existence utilization moieties followed resynthesis acylglycerophosphoethanolamine protein-dependent