Identification of UDP-glucose as an intermediate in the biosynthesis of the membrane-derived oligosaccharides of Escherichia coli.
作者： H Schulman , E P Kennedy
DOI: 10.1016/S0021-9258(17)39955-6
关键词: Uridine diphosphate glucose 、 Biochemistry 、 UTP—glucose-1-phosphate uridylyltransferase 、 Escherichia coli 、 Biosynthesis 、 Uridine Diphosphate Sugars 、 Glycogen synthase 、 Chemistry 、 Cell membrane 、 Mutant
摘要: The membrane-derived oligosaccharides of Escherichia coli constitute a closely related family containing approximately 9 glucose units variously substituted with sn-glycero-1-phosphate and phosphoethanolamine residues derived from the head groups membrane phospholipids, also succinate in O-ester linkage (Kennedy, E.P., Rumley, M.K., Schulman, H., van Golder, L.M.G. (1976) J. Biol. Chem. 251, 4208-4213). Studies mutant strains defective synthesis various nucleoside diphosphate sugars have now revealed that UDP-glucose is an essential intermediate biosynthesis these oligosaccharides. Mutants unable to synthesize do not contain significant amounts In contrast, strain ADP-glucose, glucosyl donor for glycogen E. coli, contained normal oligosaccharides, although somewhat different pattern distribution subspecies. confirmation genetic studies, pulse-label isotope tracer studies been carried out high specific activity, under conditions which comprises large fraction total radioactivity low molecular weight pool. Subsequent "chase" experiments clearly conversion higher
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