[13] 2-Acylglycerophosphoethanolamine acyltransferase/ acyl-[acyl-carrier-protein] synthetase from Escherichia coli

摘要: Publisher Summary 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase is a membrane-bound enzyme that either activates fatty acids for acyl transfer in the presence of ATP and Mg 2+ or transfers from acyl–acyl carrier protein (ACP) to 1-position lysophospholipids. 2-acyl-GPE heterodimer composed hydrophobic subunit ACP. The catalytic cycle acyltransferase/synthetase described this chapter. first step ligation acid 4 ' -phosphopantetheine sulfhydryl group ACP subunit. second reaction moiety substrate form phosphatidylethanolamine (PtdEtn). assay method based on measuring formation [14C]PtdEtn [14C]palmitic ATP, , A replica print procedure used isolate mutants lacking both acyl-ACP synthetase activities. functions only as an vivo ; its ability synthesize vitro has made it useful tool preparation defined acyl-ACPs be substrates other enzymes.