Fumonisin producing Fusarium spp. and fumonisin contamination in commercial South African maize

摘要: Fumonisins are secondary, carcinogenic me- tabolites produced primarily by Fusarium verticillioides and proliferatum on maize worldwide. The natural occurrence of fumonisin-producing spp. fumonisin contamination grain were quantified in selected cultivars from principal production areas South Africa. Grain colonization was determined using quantitative real-time PCR (qPCR) with fumonisins HPLC analysis. Kernels the 2007 samples also plated onto selective medium subsequently, split plates containing PDA & CLA. number producing spp.werequantifiedandmicroscopicallyidentifiedafter 14 days. Simple linear regression analysis used to determine relationship between target DNA, plating out method. Results indicated high infection fumonisin- concentrations warmer such as Northern Cape, North West Free State Provinces. Spearman Ranking Correlations that responses varied over localities/seasons (rs=0.42 0.64) suggesting reacted dif- ferently different environmental/inoculum conditions (disease potentials). Cultivars CRN3505 DKC80- 12B showed a degree resistance fungal infection. As colonization, Rank response vary localities/ seasons (rs=0.29 0.70). DKC80-12B LS8521B contamination.Regressionanalysis yielded significant data qPCR, but not former be better indicator potential contami- nation. Site-specific, daily maximum temperature rainfalldatawereprovidedbytheARC-Institutefor Soil Water Climate's meteorology office. No these weather parameters col- onization recorded, although tendency observed mean temperature.