Effects of cell surface charge and hydrophobicity on attachment of 16 Salmonella serovars to cantaloupe rind and decontamination with sanitizers.

摘要: Adherence of bacteria to cantaloupe rind is favored by surface irregularities such as roughness, crevices, and pits, thus reducing the ability washing or sanitizer treatments remove inactivate attached cells. In this study, we compared charge hydrophobicity two cantaloupe-related outbreak strains Salmonella Poona (RM2350 G-91-1595) those 14 additional using electrostatic hydrophobic interaction chromatography. The relative abilities 16 attach surfaces resist removal with water, chlorine (200 ppm), hydrogen peroxide (2.5%) for 5 min after a storage period up 7 days at 20 degrees C also were determined. Whole cantaloupes inoculated each pathogen 8.36 log CFU/ml, dried 1 h inside biosafety cabinet, stored, then subjected treatments. Only positive was significantly higher (P 0.05). Chlorine caused an average 3-log reduction when applied 40 postinoculation. However, 60 more postinoculation less effective (approximately 2.5-log reduction). No significant differences noted in efficacy against individual > did not differ from other tested negative cell hydrophobicity, attaching whole melon surfaces, resistant various present on rinds.