Baicalin alleviates 6-hydroxydopamine-induced neurotoxicity in PC12 cells by down-regulation of microRNA-192-5p
作者： Chunyang Kang , Libo Wang , Mingyang Kang , Xiaoyang Liu , Yao Fu
DOI: 10.1016/J.BRAINRES.2018.12.015
关键词: Molecular biology 、 PI3K/AKT/mTOR pathway 、 Baicalin 、 Kinase 、 Protein kinase B 、 Annexin 、 Propidium iodide 、 Viability assay 、 Apoptosis 、 Chemistry 、 Developmental biology 、 General Neuroscience 、 Clinical neurology
摘要: Parkinson's disease (PD), which is caused by neurodegenerative disorder, has no effective treatment until now. Baicalin was reported to have neuroprotective effects. Hence, we investigated the effects of baicalin on PD in an vitro cell model using 6-hydroxydopamine (6-OHDA)-induced neurotoxicity rat pheochromocytoma PC12 cells. cells were stimulated 6-OHDA and treated with and/or transfected miR-192-5p mimic or negative control (NC). Cell viability apoptosis examined Counting Kit-8 assay Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) analysis, respectively. The expression p62, ratio light chain (LC)3-II/LC3-I, detected qRT-PCR. All protein levels analyzed western blot. We found that significantly inhibited viability, induced autophagy, while reversed results led 6-OHDA. Moreover, negatively regulated miR-192-5p. Under treatment, transfection decreased autophagy 6-OHDA-treated compared NC. In addition, phosphorylation phosphatidylinositol 3'-kinase (PI3K) kinase B (AKT) statistically down-regulated then thereafter mimic. reduced 6-OHDA-induced injury through down-regulation miR-192-5p, as well regulation PI3K/AKT MDM-2/p53 signal pathways.
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