Bleomycin resistance as a selectable marker for transformation of the eukaryote, Dictyostelium discoideum.
作者： Andy C.M. Chang , Ruth M. Hall , Keith L. Williams
DOI: 10.1016/0378-1119(91)90312-Y
关键词: Transformation (genetics) 、 Expression cassette 、 DNA 、 Gene 、 Selectable marker 、 Molecular biology 、 Biology 、 Plasmid 、 DNA replication 、 Dictyostelium discoideum
摘要: An expression cassette was constructed, which has the bacterial bleomycin (Bm) resistance-encoding gene (ble) fused to Dictyostelium discoideum actin-6 promoter, with a segment of 3'-flanking DNA from actin-8-encoding placed downstream ble serve as transcription terminator. Plasmid pMUW161, contains this and D. plasmid Ddp2 origin replication, transformed high efficiency under Bm selection. Hence, construct is useful dominant selectable marker for discoideum.
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