Synergistic effects of 8-chlorocyclic-AMP and retinoic acid on induction of apoptosis in Ewing's sarcoma CHP-100 cells.
作者： Yoon S. Cho-Chung , Aparna R. Srivastava , Rakesh K. Srivastava
DOI:
关键词: Protein kinase A 、 Combination chemotherapy 、 Growth inhibition 、 Endocrinology 、 Tretinoin 、 Cancer cell 、 Retinoic acid 、 Cellular differentiation 、 Biology 、 Internal medicine 、 Cancer research 、 Cell growth
摘要: The enhanced expression of the regulatory subunit cyclic AMP (cAMP)-dependent protein kinase type I, RIalpha, has been correlated with cancer cell growth. Retinoic acid (RA) shown to play an important role in regulation proliferation and differentiation neoplastic cells. In present study, effects cAMP analogue 8-chlorocyclic-AMP (8-Cl-cAMP) RA (both singly combined) on growth inhibition apoptosis Ewing's sarcoma CHP-100 cells were evaluated. inhibitory 8-Cl-cAMP (9-cis-RA, 13-cis-RA, all-trans-RA) viability time dose related. degree induced by 9-cis-RA was greatest among all analogues (13-cis-RA examined. combined induction arrest at G0-G1 stage cycle, apoptosis, down-regulation cleavage poly(ADP-ribose) polymerase synergistic. conclusion, it is clear that act a synergistic fashion have potential for combination chemotherapy treatment malignant disease.
aacrjournals.org参考文章(21)
G. STANLEY MCKNIGHT, CHRISTOPHER H. CLEGG, MICHAEL D. UHLER, JOHN C. CHRIVIA, GARY G. CADD, LESLAY A. CORRELL, ANNE D. OTTEN, Analysis of the cAMP-Dependent Protein Kinase System Using Molecular Genetic Approaches Recent Progress in Hormone Research. ,vol. 44, pp. 307- 335 ,(1988) , 10.1016/B978-0-12-571144-9.50014-4
A Yen, SC rd Brooks, AA Levin, S Kazmer, Myeloid differentiation and retinoblastoma phosphorylation changes in HL-60 cells induced by retinoic acid receptor- and retinoid X receptor- selective retinoic acid analogs Blood. ,vol. 87, pp. 227- 237 ,(1996) , 10.1182/BLOOD.V87.1.227.BLOODJOURNAL871227
Grippo Jf, Lucas Da, Pemrick Sm, The retinoid receptors. Leukemia. ,vol. 8, pp. 1797- 1806 ,(1994)
J. L. Napoli, Biosynthesis and Metabolism of Retinoic Acid: Roles of CRBP and CRABP in Retinoic Acid: Roles of CRBP and CRABP in Retinoic Acid Homeostasis Journal of Nutrition. ,vol. 123, pp. 362- 366 ,(1993) , 10.1093/JN/123.SUPPL_2.362
D A Schwartz, C S Rubin, Identification and differential expression of two forms of regulatory subunits (RII) of cAMP-dependent protein kinase II in Friend erythroleukemic cells. Differentiation and 8-bromo-cAMP elicit a large and selective increase in the rate of biosynthesis of only one type of RII. Journal of Biological Chemistry. ,vol. 260, pp. 6296- 6303 ,(1985) , 10.1016/S0021-9258(18)88970-0
W N Hittelman, R Lotan, S M Lippman, W Ki Hong, Retinoid chemoprevention of aerodigestive cancer: from basic research to the clinic. Clinical Cancer Research. ,vol. 1, pp. 677- 686 ,(1995)
D. M. Gadbois, H. A. Crissman, R. A. Tobey, E. M. Bradbury, Multiple kinase arrest points in the G1 phase of nontransformed mammalian cells are absent in transformed cells. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 8626- 8630 ,(1992) , 10.1073/PNAS.89.18.8626
Y. A. Lazebnik, S. H. Kaufmann, S. Desnoyers, G. G. Poirier, W. C. Earnshaw, Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE Nature. ,vol. 371, pp. 346- 347 ,(1994) , 10.1038/371346A0
E G Krebs, J A Beavo, Phosphorylation-dephosphorylation of enzymes. Annual Review of Biochemistry. ,vol. 48, pp. 923- 959 ,(1979) , 10.1146/ANNUREV.BI.48.070179.004423
Frank A.E. Kruyt, Gert Folkers, Christina E.van den Brink, Paul T.van der Saag, A CYCLIC-AMP RESPONSE ELEMENT IS INVOLVED IN RETINOIC ACID-DEPENDENT RAR-BETA-2 PROMOTER ACTIVATION Nucleic Acids Research. ,vol. 20, pp. 6393- 6399 ,(1992) , 10.1093/NAR/20.23.6393