Platelet-Derived Growth Factor Selectively Decreases Prolactin Production in Pituitary Cells in Culture*

摘要: Platelet-derived growth factor (PDGF) markedly reduced the production of PRL without affecting GH pro-duction in GH4C1 cells. The ED50 was 6 ng/ml (2 × 10−10 M),and maximum inhibition occurred at 50 (1.5 10−9 M) PDGF. There no acute effect PDGF on release. treatment decreased after a 24-h lag; suppression rate became maximal between 24–48 h and persisted for least 14 days continued presence cell proliferation under conditions used, leucine uptake or incorporation into protein. altered morphology cells manner similar to that epidermal TRH, caused become more adherent culture substrate. actions were GH3 cells, but non-PRL-producing GC enhanced production. antagonized synthesis-stimulating activity TRH. We conclude can act directly ...