Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells
作者： Karl R Koehler , Jing Nie , Emma Longworth-Mills , Xiao-Ping Liu , Jiyoon Lee
DOI: 10.1038/NBT.3840
关键词: Biology 、 Fibroblast growth factor 、 Induced pluripotent stem cell 、 Cell culture 、 Inner ear 、 Anatomy 、 Stem cell 、 Hair cell 、 Cellular differentiation 、 Cell biology 、 Wnt signaling pathway
摘要: The derivation of human inner ear tissue from pluripotent stem cells would enable in vitro screening drug candidates for the treatment hearing and balance dysfunction may provide a source cell-based therapies ear. Here we report method differentiating to organoids that harbor functional hair cells. Using three-dimensional culture system, modulate TGF, BMP, FGF, WNT signaling generate multiple otic-vesicle-like structures single stem-cell aggregate. Over 2 months, vesicles develop into with sensory epithelia are innervated by neurons. Additionally, using CRISPR-Cas9, an ATOH1-2A-eGFP cell line detect induction demonstrate derived exhibit electrophysiological properties similar those native Our system should facilitate study development research on diseases
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