Homozygous deletion of MKK4 in ovarian serous carcinoma.

摘要: Analysis of deleted chromosomal regions in tumors has historically led to the identification tumor suppressor genes. In this study, we used digital karyotyping, a genome-wide, high-resolution technology, search for deletions ovarian serous carcinoma, most lethal gynecological malignancy women. Five purified carcinomas were analyzed by karyotyping and small interstitial at chromosome 17p identified two samples. Aligning these an overlapping region that spanned 2.4 Mb which harbored candidate gene, mitogen-activated protein kinase kinase-4 (MKK4). Dual-color FISH analysis confirmed homozygous deletion MKK4 locus both samples RT-PCR demonstrated lacked transcript expression. Loss heterozygosity occurred 24 (86%) 28 high-grade including cases with deletion. Additionally, downregulation expression was found 96 (75%) 128 as compared benign tissues. These findings suggest or reduced may contribute development carcinoma.