Telomere Length of Human Adult Bronchial Epithelium and Bronchogenic Squamous Cell Carcinoma Measured Using Tissue Quantitative Fluorescence in situ Hybridization
作者： Shinsuke Aida , Junko Aida , Kayo Hasegawa , Toshio Kumasaka , Hideyuki Shimazaki
DOI: 10.1159/000437357
关键词: Basal (phylogenetics) 、 Carcinogenesis 、 Genome instability 、 Telomere 、 Cancer 、 Lung cancer 、 Biology 、 Pathology 、 In situ hybridization 、 Telomere Homeostasis
摘要: Background: Telomeres are repetitive DNA sequences located at the ends of chromosomes. Chromosomal and genomic instability due to telomere dysfunction has been known play an important role in carcinogenesis some organs. Objectives: The aim this study was examine correlation between smoking length human bronchial epithelial cells individuals with without lung cancer. Patients Methods: We examined 68 non-lung cancer adult autopsy cases 24 surgically resected squamous cell carcinoma. Telomere lengths basal epithelium were measured using tissue quantitative fluorescence situ hybridization method expressed normalized telomere-to-centromere ratios (NTCRs). Results: autopsied included 27 current smokers (CuS), 33 never-smokers (NeS), 8 ex-smokers (ExS). NTCRs central bronchi CuS, NeS, ExS 1.515, 1.372, 1.204, respectively. telomeres CuS significantly longer than those non-CuS (NeS + ExS). When analysis conducted separately for females males, a significant difference NeS recognized only males. 1.514 1.385, Conclusions: Our findings suggest that causes telomeric elongation epithelium. Therefore, it appears mechanism smoking-related carcinomas may differ from many other which genetic aging-related shortening is assumed role.
telomer.com.tr参考文章(22)
Kaiyo Takubo, Junko Aida, Naotaka Izumiyama-Shimomura, Naoshi Ishikawa, Motoji Sawabe, Rie Kurabayashi, Hiroaki Shiraishi, Tomio Arai, Ken-Ichi Nakamura, Changes of telomere length with aging Geriatrics & Gerontology International. ,vol. 10, ,(2010) , 10.1111/J.1447-0594.2010.00605.X
Norihiro Yamaguchi, Paul A. VanderLaan, Erik Folch, David H. Boucher, Hannah M. Canepa, Michael S. Kent, Sidharta P. Gangadharan, Adnan Majid, Olivier N. Kocher, Michael A. Goldstein, Mark S. Huberman, Daniel B. Costa, Smoking status and self-reported race affect the frequency of clinically relevant oncogenic alterations in non-small-cell lung cancers at a United States-based academic medical practice Lung Cancer. ,vol. 82, pp. 31- 37 ,(2013) , 10.1016/J.LUNGCAN.2013.07.013
Ken-Ichi Nakamura, Naotaka Izumiyama-Shimomura, Kaiyo Takubo, Motoji Sawabe, Tomio Arai, Yukitoshi Aoyagi, Mutsunori Fujiwara, Eiju Tsuchiya, Yasuhito Kobayashi, Motonobu Kato, Mitsuo Oshimura, Koji Sasajima, Kei Nakachi, Comparative analysis of telomere lengths and erosion with age in human epidermis and lingual epithelium. Journal of Investigative Dermatology. ,vol. 119, pp. 1014- 1019 ,(2002) , 10.1046/J.1523-1747.2002.19523.X
W A Franklin, A F Gazdar, J Haney, I I Wistuba, F G La Rosa, T Kennedy, D M Ritchey, Y E Miller, Widely dispersed p53 mutation in respiratory epithelium. A novel mechanism for field carcinogenesis. Journal of Clinical Investigation. ,vol. 100, pp. 2133- 2137 ,(1997) , 10.1172/JCI119748
Sylvie Lantuejoul, Christophe Raynaud, Dimitri Salameire, Sylvie Gazzeri, Denis Moro-Sibilot, Jean-Charles Soria, Christian Brambilla, Elizabeth Brambilla, Telomere Maintenance and DNA Damage Responses during Lung Carcinogenesis Clinical Cancer Research. ,vol. 16, pp. 2979- 2988 ,(2010) , 10.1158/1078-0432.CCR-10-0142
N. Kim, M. Piatyszek, K. Prowse, C. Harley, M. West, P. Ho, G. Coviello, W. Wright, S. Weinrich, J. Shay, Specific association of human telomerase activity with immortal cells and cancer Science. ,vol. 266, pp. 2011- 2015 ,(1994) , 10.1126/SCIENCE.7605428
Junko Aida, Akira Yokoyama, Naotaka Izumiyama, Ken-ichi Nakamura, Naoshi Ishikawa, Steven S Poon, Mutsunori Fujiwara, Motoji Sawabe, Masaaki Matsuura, Tomio Arai, Kaiyo Takubo, Alcoholics show reduced telomere length in the oesophagus. The Journal of Pathology. ,vol. 223, pp. 410- 416 ,(2011) , 10.1002/PATH.2817
Kaiyo Takubo, Naotaka Izumiyama-Shimomura, Naoko Honma, Motoji Sawabe, Tomio Arai, Motonobu Kato, Mitsuo Oshimura, Ken-Ichi Nakamura, Telomere lengths are characteristic in each human individual Experimental Gerontology. ,vol. 37, pp. 523- 531 ,(2002) , 10.1016/S0531-5565(01)00218-2
Tracy M. Bryan, Anna Englezou, Luciano Dalla-Pozza, Melissa A. Dunham, Roger R. Reddel, EVIDENCE FOR AN ALTERNATIVE MECHANISM FOR MAINTAINING TELOMERE LENGTH IN HUMAN TUMORS AND TUMOR-DERIVED CELL LINES Nature Medicine. ,vol. 3, pp. 1271- 1274 ,(1997) , 10.1038/NM1197-1271
Kiyoshi Shibuya, Takehiko Fujisawa, Hidehisa Hoshino, Masayuki Baba, Yukio Saitoh, Toshihiko Iizasa, Yasuo Sekine, Makoto Suzuki, Kenzo Hiroshima, Hidemi Ohwada, Increased telomerase activity and elevated hTERT mRNA expression during multistage carcinogenesis of squamous cell carcinoma of the lung. Cancer. ,vol. 92, pp. 849- 855 ,(2001) , 10.1002/1097-0142(20010815)92:4<849::AID-CNCR1392>3.0.CO;2-4