193 nm ultraviolet photodissociation mass spectrometry for the structural elucidation of lipid A compounds in complex mixtures.
作者： John P. O’Brien , Brittany D. Needham , Jeremy C. Henderson , Emily M. Nowicki , M. Stephen Trent
DOI: 10.1021/AC403796N
关键词: Orbitrap 、 Mass spectrometry 、 Tandem mass spectrometry 、 Chemistry 、 Glycosidic bond 、 Photochemistry 、 Collision-induced dissociation 、 Dissociation (chemistry) 、 Analytical chemistry 、 Ion 、 Photodissociation
摘要: Here we implement ultraviolet photodissociation (UVPD) in an online liquid chromatographic tandem mass spectrometry (MS/MS) strategy to support analysis of complex mixtures lipid A combinatorially modified during development vaccine adjuvants. UVPD at 193 nm was utilized characterize the structures and fragment ion types from Escherichia coli, Vibrio cholerae, Pseudomonas aeruginosa using Orbitrap spectrometer. The ions generated by were compared those collision induced dissociation (CID) higher energy (HCD) with respect precursor charge state. afforded widest array including acyl chain C–O, C–N, C–C bond cleavages glycosidic C–O cross ring cleavages, thus providing most comprehensive structural A. exhibited virtually no dependence on state best determining structure l...
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