252Cf plasma desorption mass spectrometry applied to the analysis of underivatized rough-type endotoxin preparations.

摘要: Plasma desorption mass spectrometry has recently been used with success to characterize native, underivatized Re- Rc-type endotoxins in terms of their constituent lipopolysaccharides. The spectra give masses for the major molecular species lipopolysaccharide present from which probable compositions could be inferred using overall composition determined by chemical analyses. Moreover, relative intensities signals are roughly proportional abundance corresponding species. Native Rc-, Rb-, and Ra-type enterobacterial 5-10 core sugar units have rendered amenable plasma-desorption analysis improvement solubility use cellobiose as an additive. four Salmonella Escherichia endotoxin preparations demonstrated heterogeneity acylation phosphorylation. Since these sources critical many biological activities, underline need define each preparation structure-function studies.