Deformability-based red blood cell separation in deterministic lateral displacement devices-A simulation study.
作者： Timm Krüger , David Holmes , Peter V. Coveney
DOI: 10.1063/1.4897913
关键词: Lateral extension 、 Lateral displacement 、 Chemistry 、 Red blood cell 、 Biomedical engineering 、 Microfluidics 、 Cellular biophysics 、 Biophysics
摘要: We show, via three-dimensional immersed-boundary-finite-element-lattice-Boltzmann simulations, that deformability-based red blood cell (RBC) separation in deterministic lateral displacement (DLD) devices is possible. This due to the deformability-dependent extension of RBCs and enables us predict a priori which will be displaced given DLD geometry. Several diseases affect deformability human cells. Malaria-infected RBCs, for example, tend become stiffer than their healthy counterparts. It therefore desirable design microfluidic can detect based on cells' fingerprint, rather preparing samples using expensive time-consuming biochemical preparation steps. Our findings should helpful development new methods sorting cells particles by deformability.
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sci-hub.se 参考文章(38)
S Shrivastava, J Tang, Large deformation finite element analysis of non-linear viscoelastic membranes with reference to thermoforming Journal of Strain Analysis for Engineering Design. ,vol. 28, pp. 31- 51 ,(1993) , 10.1243/03093247V281031
Lotien Richard Huang, Edward C Cox, Robert H Austin, James C Sturm, Continuous particle separation through deterministic lateral displacement. Science. ,vol. 304, pp. 987- 990 ,(2004) , 10.1126/SCIENCE.1094567
David W. Inglis, John A. Davis, Robert H. Austin, James C. Sturm, Critical particle size for fractionation by deterministic lateral displacement Lab on a Chip. ,vol. 6, pp. 655- 658 ,(2006) , 10.1039/B515371A
Nicole Pamme, Continuous flow separations in microfluidic devices Lab on a Chip. ,vol. 7, pp. 1644- 1659 ,(2007) , 10.1039/B712784G
Yuchao Chen, Peng Li, Po-Hsun Huang, Yuliang Xie, John D. Mai, Lin Wang, Nam-Trung Nguyen, Tony Jun Huang, Rare cell isolation and analysis in microfluidics Lab on a Chip. ,vol. 14, pp. 626- 645 ,(2014) , 10.1039/C3LC90136J
David W. Inglis, John A. Davis, Thomas J. Zieziulewicz, David A. Lawrence, Robert H. Austin, James C. Sturm, Determining blood cell size using microfluidic hydrodynamics. Journal of Immunological Methods. ,vol. 329, pp. 151- 156 ,(2008) , 10.1016/J.JIM.2007.10.004
Nezihi Murat Karabacak, Philipp S Spuhler, Fabio Fachin, Eugene J Lim, Vincent Pai, Emre Ozkumur, Joseph M Martel, Nikola Kojic, Kyle Smith, Pin-i Chen, Jennifer Yang, Henry Hwang, Bailey Morgan, Julie Trautwein, Thomas A Barber, Shannon L Stott, Shyamala Maheswaran, Ravi Kapur, Daniel A Haber, Mehmet Toner, Microfluidic, marker-free isolation of circulating tumor cells from blood samples Nature Protocols. ,vol. 9, pp. 694- 710 ,(2014) , 10.1038/NPROT.2014.044
Mohamed Al-Fandi, Mohammad Al-Rousan, Mohammad A.K. Jaradat, Lina Al-Ebbini, New design for the separation of microorganisms using microfluidic deterministic lateral displacement Robotics and Computer-integrated Manufacturing. ,vol. 27, pp. 237- 244 ,(2011) , 10.1016/J.RCIM.2010.06.003
Jochen Guck, Stefan Schinkinger, Bryan Lincoln, Falk Wottawah, Susanne Ebert, Maren Romeyke, Dominik Lenz, Harold M. Erickson, Revathi Ananthakrishnan, Daniel Mitchell, Josef Käs, Sydney Ulvick, Curt Bilby, Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence. Biophysical Journal. ,vol. 88, pp. 3689- 3698 ,(2005) , 10.1529/BIOPHYSJ.104.045476
T. Krüger, F. Varnik, D. Raabe, Efficient and accurate simulations of deformable particles immersed in a fluid using a combined immersed boundary lattice Boltzmann finite element method Computers & Mathematics With Applications. ,vol. 61, pp. 3485- 3505 ,(2011) , 10.1016/J.CAMWA.2010.03.057