Integrated Microfluidic System for Size-Based Selection and Trapping of Giant Vesicles

摘要: Vesicles composed of phospholipids (liposomes) have attracted interest as artificial cell models and been widely studied to explore lipid–lipid lipid–protein interactions. However, the size dispersity liposomes prepared by conventional methods was a major problem that inhibited their use in high-throughput analyses based on monodisperse liposomes. In this study, we developed an integrative microfluidic device enables both size-based selection trapping This consists hydrodynamic channels series, which made it possible successfully produce array more than 60 from polydisperse liposome suspension with narrow distribution (the coefficient variation less 12%). We observed size-dependent response sequential osmotic stimuli, had not clarified so far, using device. Our will be powerful tool facilitate statistical analysis o...