Internal standards for quantitative competitive pcr

摘要: The present invention relates to a method for constructing internal standards used in competitive polymerase chain reaction (PCR) determining the amount of target nucleic acid sequence sample. comprises steps a) digesting cloned with cohesive-end generating restriction endonuclease; b) filling endonuclease cohesive-ends step by Klenow fragment DNA polymerase; and c) blunt-end ligating form standard. standard differs from such that recognition site is destroyed adding sufficient contiguous bases size difference between can be detected gel electrophoresis.