Quantitation of nucleic acids using the polymerase chain reaction
作者： Michael V. Doyle , Alice M. Wang , David F. Mark
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摘要: The present invention provides a method for determining the amount of target acid segment in sample by polymerase chain reaction. involves simultaneous amplification or nucleic and an internal standard segment. amplified DNA from each is determined compared to curves determine prior amplification. especially preferred quantity specific mRNA species biological sample. Additionally, provided useful quantitation multiple species.
lens.org 参考文章(8)
D. Rappolee, D Mark, M. Banda, Z Werb, Wound macrophages express TGF-alpha and other growth factors in vivo: analysis by mRNA phenotyping. Science. ,vol. 241, pp. 708- 712 ,(1988) , 10.1126/SCIENCE.3041594
Jamel Chelly, Jean-Claude Kaplan, Pascal Maire, Sophie Gautron, Axel Kahn, Transcription of the dystrophin gene in human muscle and non-muscle tissues Nature. ,vol. 333, pp. 858- 860 ,(1988) , 10.1038/333858A0
Michael Becker-André, Klaus Hahlbrock, Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by a PCR aided transcript titration assay (PATTY). Nucleic Acids Research. ,vol. 17, pp. 9437- 9446 ,(1989) , 10.1093/NAR/17.22.9437
Stephen Joel Scharf, Norman Arnheim, Henry Anthony Erlich, Kary Banks Mullis, Glenn Thomas Horn, Randall Keichi Saiki, Process for amplifying, detecting, and/or-cloning nucleic acid sequences ,(1986)
Frances C. Lawyer, Randall K. Saiki, Susanne Stoffel, David H. Gelfand, Purified thermostable enzyme ,(1989)
Kary Banks Mullis, Process for amplifying nucleic acid sequences ,(1986)
Thomas Raymond Gingeras, Ulrich Merten, Deborah Yantis Kwoh, Transcription-based nucleic acid amplification/detection systems ,(1988)
Gary Gilliland, Steven Perrin, H. Franklin Bunn, 8 – COMPETITIVE PCR FOR QUANTITATION OF mRNA PCR Protocols#R##N#A Guide to Methods and Applications. pp. 60- 69 ,(1990) , 10.1016/B978-0-12-372180-8.50012-3