Purified thermostable enzyme
作者： Frances C. Lawyer , Randall K. Saiki , Susanne Stoffel , David H. Gelfand
DOI:
关键词: DNA polymerase 、 Enzyme 、 Sequence (biology) 、 Recombinant DNA 、 Nucleotide 、 Thermus aquaticus 、 Nucleic acid sequence 、 Biology 、 Recombinant enzyme 、 Biochemistry
摘要: Recombinant DNA sequences encoding a thermostable polymerase from Thermus aquaticus can be used to produce recombinant protein with molecular weight of about 86,000-95,000 daltons. The enzyme in temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified quantity an existing the aid selected primers and nucleotide triphosphates. preferably stored buffer containing non-ionic detergents that lends stability enzyme.
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