Probe for detecting polymorphism in egfr genes, and usage therefor
作者： Moeko Ijuin
DOI:
关键词: Base (exponentiation) 、 Polymorphism (computer science) 、 Base sequence 、 Sequence (medicine) 、 Oligonucleotide 、 Polymorphism Detection 、 Molecular biology 、 Base Number 、 Gene 、 Biology
摘要: Provided is a polymorphism detection probe that can easily and very reliably discern in EGFR genes. Also provided method using said probe. The characterized by containing at least one of the following: (P1) an oligonucleotide between 22 50 bases long comprises base sequence complementary to numbers 334 355 number 1, wherein 3′-end region; (P2) comprising (P1).
lens.org参考文章(17)
Huijuan Qin, Jiasen Xu, Gang Yu, Guoqiang Li, 秦会娟, 余刚, 许嘉森, 李国强, Liquid chip for detecting egfr gene mutations ,(2011)
Ji-Dung Luo, Chiuan-Chian Chiou, Methods and kits for the detection of nucleotide mutations using peptide nucleic acid as both PCR clamp and sensor probe ,(2007)
David H. Gelfand, Susanne Stoffel, Frances C. Lawyer, Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase ,(1990)
I. A. Kofiadi, D. V. Rebrikov, [Methods for detecting single nucleotide polymorphisms: allele-specific PCR and hybridization with oligonucleotide probe]. Russian Journal of Genetics. ,vol. 42, pp. 16- 26 ,(2006) , 10.1134/S1022795406010029
William Pao, Vincent A Miller, Katerina A Politi, Gregory J Riely, Romel Somwar, Maureen F Zakowski, Mark G Kris, Harold Varmus, Acquired Resistance of Lung Adenocarcinomas to Gefitinib or Erlotinib Is Associated with a Second Mutation in the EGFR Kinase Domain PLoS Medicine. ,vol. 2, pp. e73- ,(2005) , 10.1371/JOURNAL.PMED.0020073
Yasushi Yatabe, Toyoaki Hida, Yoshitsugu Horio, Takayuki Kosaka, Takashi Takahashi, Tetsuya Mitsudomi, A Rapid, Sensitive Assay to Detect EGFR Mutation in Small Biopsy Specimens from Lung Cancer The Journal of Molecular Diagnostics. ,vol. 8, pp. 335- 341 ,(2006) , 10.2353/JMOLDX.2006.050104
Yi-Shuian Huang, Ying-Xin Wang, Neil X. Krueger, Martin Fuchs, Devices and methods for enrichment and alteration of circulating tumor cells and other particles ,(2005)
Philip S Bernard, Carl T Wittwer, Real-Time PCR Technology for Cancer Diagnostics Clinical Chemistry. ,vol. 48, pp. 1178- 1185 ,(2002) , 10.1093/CLINCHEM/48.8.1178
Jin Li, Lilin Wang, Pasi A Jänne, G Mike Makrigiorgos, Coamplification at Lower Denaturation Temperature-PCR Increases Mutation-Detection Selectivity of TaqMan-Based Real-Time PCR Clinical Chemistry. ,vol. 55, pp. 748- 756 ,(2009) , 10.1373/CLINCHEM.2008.113381
Francine Perler, Rebecca Kucera, William E. Jack, Donald G. Comb, Purified thermostable dna polymerase obtainable from thermococcus litoralis ,(1991)