A Rapid, Sensitive Assay to Detect EGFR Mutation in Small Biopsy Specimens from Lung Cancer

摘要: It has been demonstrated that lung cancers, specifically a subset of pulmonary adenocarcinomas, with epidermal growth factor receptor (EGFR) mutation are highly sensitive to EGFR-targeted drugs. Therefore, rapid, assay for detection using routine pathological specimens is demanded in clinical practice predict the response. We therefore developed new detecting EGFR only paraffin section small biopsy specimen. The method was very sensitive, as few 5% cancer cells background normal cells, results usually being obtained within 4 hours. Furthermore, it accurate, shown by high concordance reverse transcriptase-polymerase chain reaction-coupled direct sequencing (186 195, 95%). practical application this 29 cases treated gefitinib resulted prediction rate: 10 11 responders were be positive mutation, and all patients progressive disease negative. In addition, at codon 790, conferring resistance, successfully analyzed similar manner. conclusion, sections without tumor cell-enrichment procedure quite useful select treatment choice practice.