Primer compositions and kits for detecting hepatitis B virus
作者： Isa K. Mushahwar , Larry Birkenmeyer
DOI:
关键词: Primer (molecular biology) 、 Hepatitis B virus 、 Oligonucleotide Primer 、 Hapten 、 Biology 、 Oligonucleotide primers 、 Sample (material) 、 Chromatography 、 Nucleic acid sequence 、 Sequence (medicine) 、 Molecular biology
摘要: The present invention provides a method for quantitatively detecting the amount of target nucleic acid sequence which may be in test sample. A sample contain comprising sequences X and Y is contacted with two primer sets, first set being specific second Y. also at same time an internal standard IS, substantially derived from combination sequences, its corresponding oligonucleotide primers. Haptens are associated sets such way that amplified products detected by capture on solid phase to anti-hapten reagents attached. signal ratio (X+Y)/S determined quantitate contained
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