The Drosophila melanogaster Toll Pathway Participates in Resistance to Infection by the Gram-Negative Human Pathogen Pseudomonas aeruginosa

摘要: Pseudomonas aeruginosa is a gram-negative pathogen that infects immunocompromised and cystic fibrosis patients. The molecular basis of the host-P. interaction effect specific P. virulence factors on various components innate immunity pathways are largely unknown. We examine interactions between response in Drosophila melanogaster model system to reveal importance Toll signaling pathway resistance infection by human isolate PA14. Using two PA14-isogenic mutants plcS dsbA, we show loss-of-function Spatzle, extracellular ligand Toll, Dorsal Dif, NF-κB-like transcription factors, allow increased infectivity within fly tissues. In contrast, constitutively active mutant Cactus, an IκB-like factor inhibits signaling, reduce infectivity. Our finding activity required restrict provides direct vivo evidence for function adult immunity. Additionally, our results provide future studies into pathway, which functionally conserved flies humans.