Invasiveness of T-Cell Hybridomas in Vitro and Their Metastatic Potential in Vivo

摘要: BW5147 lymphoma cells, which are noninvasive and nonmetastatic, were fused with normal T-lymphocytes. The invasiveness of the generated T-cell hybridomas was tested in hepatocyte cultures, their metastatic potential by tail vein injection. A total 29 from alloantigen-activated T-cells all found to be invasive. One these cell lines rapidly lost culture. Most nonstimulated spleen also invasive, but 5 27 not. Six invasive four injected into syngeneic mice. All caused extensive widespread tumor growth, particularly liver, usually severalfold enlarged; spleen; kidneys; ovaries. In contrast hybrids, tumorigenic upon s.c. injection, did not form any metastases. We conclude that properties derived T-cells, when introduced T-lymphoma cause them become as well metastatic. Furthermore for this type measured cultures appears closely associated ability colonize organs bloodstream.