Zebrafish as a model organism to study host-pathogen interactions.

摘要: Abstract Zebrafish have been extensively used in biomedical research as a model to study vertebrate development but it is only recently that has also adopted into varied fields such immunology and host–pathogen interactions. rapid life cycle, small size the adults exhibit no territorial behavior relatively dense cages. Under standard conditions each female lays an average of hundred eggs per clutch, providing large number larvae week. Their transparency during early stages allows real time visualization different organs, which makes them especially suitable for bacterial Traditionally, these studies technically challenging higher organisms, given loss control over bacteria once pathogen infects its host. Here we describe emerging approach monitor Salmonella typhimurium infection progression using vivo fluorescence upon parenteral infection. We engineered with Cascade expression system; efficient method voluntarily activate heterologous gene at any point inside macrophages, non-toxic inducer.