Enzymic degradation of polymers. I. Viscometric method for the determination of enzymic activity.

作者: Karl Erik Almin , Karl-Erik Eriksson

DOI: 10.1016/0005-2744(67)90028-9

关键词: Enzymic degradationChromatographySubstrate (chemistry)CellulasePolymerChemistryOrganic chemistry

摘要: Abstract A viscometric method for the determination of enzymic activity has been developed from a theoretical and semiempirical point view. The makes possible units polymer degrading enzymes in absolute terms. It adapted to cellulases with CM-cellulose as substrate.

参考文章(7)
Göran Pettersson, Jerker Porath, [103] A cellulolytic enzyme from Penicillium notatum Methods in Enzymology. ,vol. 8, pp. 603- 607 ,(1966) , 10.1016/0076-6879(66)08109-6
Michael Somogyi, NOTES ON SUGAR DETERMINATION Journal of Biological Chemistry. ,vol. 195, pp. 19- 23 ,(1926) , 10.1016/S0021-9258(18)84465-9
K. Hess, W. Philippoff, Über die Konzentrationsabhängigkeit der Zähigkeit bei organischen Hochpolymeren European Journal of Inorganic Chemistry. ,vol. 70, pp. 639- 665 ,(1937) , 10.1002/CBER.19370700411
Frank Baker, CLXXX.—The viscosity of cellulose nitrate solutions Journal of The Chemical Society, Transactions. ,vol. 103, pp. 1653- 1675 ,(1913) , 10.1039/CT9130301653
Maurice L. Huggins, THE VISCOSITY OF DILUTE SOLUTIONS OF LONG-CHAIN MOLECULES, IV. DEPENDENCE ON CONCENTRATION Journal of the American Chemical Society. ,vol. 64, pp. 2716- 2716 ,(1942) , 10.1021/JA01263A056
D.R. Whitaker, Hydrolysis of a series of β-1,4′-oligoglucosides by Myrothecium verrucaria cellulase Archives of Biochemistry and Biophysics. ,vol. 53, pp. 439- 449 ,(1954) , 10.1016/0003-9861(54)90425-7
A. Fredrik, V. Eriksson, Sven Lindvall, Studies on a new cellulase preparation from Penicillium. I. Method of determining enzymatic activity. Journal of Pharmacy and Pharmacology. ,vol. 11, pp. 747- 755 ,(2011) , 10.1111/J.2042-7158.1959.TB12622.X