[103] A cellulolytic enzyme from Penicillium notatum

摘要: Publisher Summary This chapter discusses the synthesis of a cellulolytic enzyme from Penicillium notatum . The assay is based on increase in reducing groups following incubation carboxymethylcellulose (CMC) with enzyme. For purposes purification and preliminary characterization it sufficient to introduce an arbitrary unit. One unit defined as activity necessary produce power corresponding 1.00 absorbancy at 640 mμ. specific may then be expressed units per solutions 280 catalyzes hydrolytic cleavage soluble cellulose derivatives such carboxymethylcellulose, hydroxyethylcellulose, ethylhydroxyethylcellulose. Cellodextrins are also hydrolyzed by In contrast ordinary cotton cellulose, swollen or regenerated celluloses attacked. can kept room temperature for least week pH 4.5–6.5 without any detectable loss activity, however should preferably stored close 0°. reagents used, procedure followed, steps involved described chapter.