Chromatoprobe as a sample-sparing technique for residual solvent analysis of drug discovery candidates by gas chromatography.

摘要: Abstract In drug discovery research, residual solvent measurement is an integral part of purity analysis for synthesis a candidate before it used toxicity testing. This usually carried out using gas chromatography (GC) with direct injection sample introduction. method requires testing compounds to be soluble at high concentrations (>50 mg/mL, in DMSO) achieve acceptable sensitivity, hurdle which not always achievable some samples such as cyclic peptides and oligonucleotides. To overcome the limitation associated approach, new Chromatoprobe thermal extraction device was developed quantifying solvents compounds. only consumes significantly less material (less than 1 mg), but also shows higher sensitivity approach. addition, because no diluent required extraction, all can detected measured without further optimization. our study, we compared data from GC solid introduction those seven in-house samples. Our results showed good agreement between these two methods. Thus, provided sample-sparing alternative discovery. particularly useful that are available limited amounts, poorly soluble, and/or unstable diluents method.