Phylogenetic characterization of bacteria in the gut of house flies (Musca domestica L.).

摘要: House flies ( M usca domestica L.) are cosmopolitan, ubiquitous, synanthropic insects that serve as mechanical or biological vectors for various microorganisms. To fully assess the role of house in epidemiology human diseases, it is essential to understand diversity microbiota harbored by natural fly populations. This study aimed identify gut bacteria both culture-dependent and culture-independent approaches. A total 102 bacterial strains were isolated from 65 collected public places including a garden, park, garbage/dump area, toilet, hospital, restaurant/canteen, mutton shop/market, house/human habitation. Molecular phylogenetic analyses placed these isolates into 22 different genera. The majority identified known potential pathogens genera K lebsiella , eromonas S higella organella P rovidencia taphylococcus . Culture-independent methods involved construction 16S rRNA gene clone library, sequence supported culture recovery results. However, additional taxa not determined via revealed using this methodology included members classes lphaproteobacteria D eltaproteobacteria phylum B acteroidetes Here, we show an environmental reservoir vast number species, which may have impacts on vector pathogen transmission.