作者: L. Přenosilová , Z. Křesinová , A. Slavíková Amemori , T. Cajthaml , K. Svobodová
关键词: Trametes 、 Mycelium 、 Irpex lacteus 、 Microbiology 、 Fungal protein 、 Laccase 、 Trametes versicolor 、 Biology 、 Manganese peroxidase 、 Biochemistry 、 Peroxidase
摘要: Fungal, ligninolytic enzymes have attracted a great attention for their bioremediation capabilities. A deficient knowledge of regulation enzyme production, however, hinders the use fungi in applications. In this work, transcriptional analyses laccase and manganese peroxidase (MnP) production by two white rots was combined with determination pI evaluation 17α-ethinyloestradiol (EE2) degradation to study mechanisms used during EE2 degradation. cultures Trametes versicolor addition caused an increase activity maximum 34.2 ± 6.7 U g−1 dry mycelia that observed after 2 days cultivation. It corresponded 4.9 times higher transcription levels laccase-encoding gene (lacB) were detected at same time. Simultaneously, values fungal laccases altered response treatment. Like T. versicolor, Irpex lacteus also able remove 10 mg l−1 within 3 While I. lacteus MnP MnP later phase suggests another metabolic role but