Laccase activity profiling and gene expression in PCB-degrading cultures of Trametes versicolor

摘要: Abstract This study examined laccase production by the fungus Trametes versicolor while it was degrading Delor 103, a commercial mixture of polychlorinated biphenyls (PCBs). 103 (10 mg l−1) caused significant increase in activity culture supernatants nitrogen-limited medium cultures (the maximum 1439 U g−1 42 times that controls) where induction effect suppressed malt extract – glucose medium. Similarly, stimulation depended on growth phase fungal cultures. Higher activities developed treated with non-growing correlated an up to 2.6-fold greater gene expression, which analysed quantitative polymerase chain reaction (qPCR). Simultaneously, laccases different pIs were formed response treatment. When analysing expression degenerated primers and denaturing gradient gel electrophoresis, differences between transcript profiles 103-treated untreated control observed. Despite all production, no PCB removal observed T. versicolor makes connection degradation uncertain.