A comparative study on the enhancement efficacy of specific and non-specific iron chelators for protoporphyrin IX production and photosensitization in HaCat cells
作者： Yumin Xia , Yingying Huang , Longde Lin , Xiaoming Liu , Shan Jiang
DOI: 10.1007/S11596-009-0619-X
关键词: Photodynamic therapy 、 Fluorescence 、 Programmed cell death 、 Apoptosis 、 Chelation 、 HaCaT 、 Protoporphyrin IX 、 Molecular biology 、 Chemistry 、 Ethylenediaminetetraacetic acid 、 Biochemistry 、 Genetics 、 Biomaterials 、 Biomedical engineering
摘要: The iron chelators can be utilized in target cells to improve 5-aminolaevulinic acid (ALA)-based photodynamic therapy (PDT). purpose of this study is compare the effect two kinds chelators, desferrioxamine (DFO) and ethylenediaminetetraacetic (EDTA) on enhancement ALA-PDT. HaCat were cultured medium containing 2.0 mmol/L ALA 0.5 DFO or EDTA. After 3-h incubation dark, concentration cellular protoporphyrin IX (PpIX) was detected by high performance liquid chromatography (HPLC), fluorescence PpIX observed at 630 nm emission under confocal laser scanning microscope. For PDT, irradiated using 632.8 laser, fractions apoptotic necrotic flow cytometrically assayed. Related differences morphology ultrastructure Ha-Cat optical microscope transmission electron Compared with alone, addition EDTA increased fluorescent density PpIX, also cell death ratio after PDT. PDT plus produced highest level, greatest most severe structural damage cells. It concluded that both could enhance ALA-based production non-specific chelator EDTA, specific chelator, DFO, showed more potential for enhancement.
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