Plant Protoplast Enucleation by Density Gradient Centrifugation

摘要: A number of different methods have been developed for the enucleation cells, particularly mammalian cells. Centrifugation cells adhering to a surface may lead nucleus being drawn out from cytoplasm, resulting in formation an enucleated cell or cytoplast and surrounded by thin layer cytoplasm bounded plasma membrane (mini-cell). In those cases where do not adhere sufficiently firmly surface, has achieved centrifugation density gradients. This method takes advantage differing densities cytoplasm. When are centrifuged at high speed suitable gradient, tend float regions gradient matching their own densities. If distance between with corresponding that is relatively long, considerable stretching forces generated. Under optimum conditions, this force usually sufficient enucleate percentage The use gradients high-speed generating cytoplasts mini-cells much less explored plant than