Autocrine signaling through ATP release represents a novel mechanism for cell volume regulation
作者： Y. Wang , R. Roman , S. D. Lidofsky , J. G. Fitz
关键词: Cell membrane 、 Osmotic shock 、 Cell biology 、 Extracellular 、 Apyrase 、 Autocrine signalling 、 Purinergic receptor 、 Chloride channel 、 Chemistry 、 Patch clamp
摘要: Recovery of cell volume in response to osmotic stress is mediated part by increases the Cl- permeability plasma membrane. These studies evaluate hypothesis that ATP release and autocrine stimulation purinergic (P2) receptors couple opening channels. In HTC rat hepatoma cells, swelling induced hypotonic exposure increased membrane current density 44.8 +/- 7.1 pA/pF at -80 mV. Both rate recovery increase were inhibited presence hydrolase apyrase (3 units/ml) or P2 receptor blockers suramin Reactive Blue 2 (10-100 microM). Cell also stimulated ATP. Hypotonic concentration effluent perfused cells 170 36 nM a nucleotidase inhibitor (P < 0.01). whole-cell recordings with as charge carrier, approximately 30-fold 16.5 10.4 pA/pF. findings indicate lead efflux through conductive pathway consistent channel, extracellular required for from swelling. may function an factor couples channels receptors.
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D Häussinger, C Hallbrucker, N Saha, F Lang, W Gerok, Cell volume and bile acid excretion Biochemical Journal. ,vol. 288, pp. 681- 689 ,(1992) , 10.1042/BJ2880681
E R Lazarowski, T K Harden, Identification of a uridine nucleotide-selective G-protein-linked receptor that activates phospholipase C. Journal of Biological Chemistry. ,vol. 269, pp. 11830- 11836 ,(1994) , 10.1016/S0021-9258(17)32648-0
R. S. Chari, S. M. Schutz, J. E. Haebig, G. H. Shimokura, P. B. Cotton, J. G. Fitz, W. C. Meyers, Adenosine nucleotides in bile American Journal of Physiology-gastrointestinal and Liver Physiology. ,vol. 270, ,(1996) , 10.1152/AJPGI.1996.270.2.G246
D A Ausiello, I L Reisin, A G Prat, H F Cantiello, R J Gregory, E H Abraham, J F Amara, The cystic fibrosis transmembrane conductance regulator is a dual ATP and chloride channel. Journal of Biological Chemistry. ,vol. 269, pp. 20584- 20591 ,(1994) , 10.1016/S0021-9258(17)32033-1
K Kirk, J.C. Ellory, J.D. Young, Transport of organic substrates via a volume-activated channel. Journal of Biological Chemistry. ,vol. 267, pp. 23475- 23478 ,(1992) , 10.1016/S0021-9258(18)35862-9
S.D. Lidofsky, M.H. Xie, A. Sostman, B.F. Scharschmidt, J.G. Fitz, Vasopressin increases cytosolic sodium concentration in hepatocytes and activates calcium influx through cation-selective channels. Journal of Biological Chemistry. ,vol. 268, pp. 14632- 14636 ,(1993) , 10.1016/S0021-9258(18)82380-8
F Okajima, Y Tokumitsu, Y Kondo, M Ui, P2-purinergic receptors are coupled to two signal transduction systems leading to inhibition of cAMP generation and to production of inositol trisphosphate in rat hepatocytes. Journal of Biological Chemistry. ,vol. 262, pp. 13483- 13490 ,(1987) , 10.1016/S0021-9258(19)76452-7
Jonathan A. Cohn, Theresa V. Strong, Marina R. Picciotto, Angus C. Nairn, Francis S. Collins, J.Gregory Fitz, Localization of the cystic fibrosis transmembrane conductance regulator in human bile duct epithelial cells Gastroenterology. ,vol. 105, pp. 1857- 1864 ,(1993) , 10.1016/0016-5085(93)91085-V
P. S. Jackson, K. Strange, Volume-sensitive anion channels mediate swelling-activated inositol and taurine efflux American Journal of Physiology-cell Physiology. ,vol. 265, ,(1993) , 10.1152/AJPCELL.1993.265.6.C1489
Erik M. Schwiebert, Marie E. Egan, Tae-Ho Hwang, Stephanie B. Fulmer, Sandra S. Allen, Garry R. Cutting, William B. Guggino, CFTR regulates outwardly rectifying chloride channels through an autocrine mechanism involving ATP. Cell. ,vol. 81, pp. 1063- 1073 ,(1995) , 10.1016/S0092-8674(05)80011-X