Multiplexed MRM-based protein quantification of putative prognostic biomarkers for chronic kidney disease progression in plasma.

摘要: Current diagnostic measures for Chronic Kidney Disease (CKD) include detection of reduced estimated glomerular filtration rate (eGFR) and albuminuria, which have suboptimal accuracies in predicting disease progression. The complexity heterogeneity underscore the need multiplex quantification different markers. goal this study was to determine association six previously reported CKD-associated plasma proteins [B2M (Beta-2-microglobulin), SERPINF1 (Pigment epithelium-derived factor), AMBP (Protein AMBP), LYZ (Lysozyme C), HBB (Hemoglobin subunit beta) IGHA1 (Immunoglobulin heavy constant alpha 1)], as measured a format, with kidney function, outcome. Antibody-free, multiple reaction monitoring mass spectrometry (MRM) assays were developed, characterized their analytical performance, used analysis 72 samples from patient cohort longitudinal follow-up. MRM significantly correlated (Rho = 0.5–0.9) results respective ELISA. Five [AMBP, B2M, LYZ, SERPINF1] associated eGFR, three former also unfavorable combination these markers provided stronger associations outcome (p < 0.0001) compared individual Collectively, our describes assay absolute verification described putative CKD prognostic markers, laying groundwork further use prospective validation studies.