MACROPHAGES INDUCE CELLULAR IMMUNITY BY ACTIVATING TH1 CELL RESPONSES AND SUPPRESSING TH2 CELL RESPONSES

摘要: Differentiation of naive CD4+ T cells (Th0) into Th1 or Th2 determines whether antigen will raise a cellular humoral immune response. The maturation pathway chosen by the Th0 cell is often decisive for outcome disease and depends among others on (co-)stimulatory attributes APC nature abundance cytokines provided microenvironment. In this study, we used macrophages, loaded ex vivo with antigen, inciting activation differentiation in vivo. macrophages were derived from clonal, immortalized population that both functionally phenotypically expressed features characteristic mature macrophages. Injection syngeneic mice IFN-gamma-treated, Ag-loaded induced primary response, indicated occurrence proliferative response vitro after restimulation splenocytes Ag. Analysis accompanying cytokine secretion revealed high numbers IFN-gamma-producing only few IL-4-secreting cells. This dominance had functional implications, reflected titer cell-dependent IgG2 Abs absence IgG1, immunity. Moreover, administration to an ongoing Th1/Th2 resulted complete suppression IgG1 production, whereas levels remained unaffected. These results demonstrate exert activity organism, strongly skew responses immunity, addition suppress already generated Th2-dependent thus characterizing these as Th1-oriented APC.