Graft rejection by cytolytic T cells. Specificity of the effector mechanism in the rejection of allogeneic marrow.

摘要: Cellular effector mechanisms of allograft rejection remain incompletely described. Characterizing the foreign-marrow allografts rather than solid-organ grafts has advantage that cellular composition marrow graft, as a single cell suspension, can be altered to include components with differing antigen expression. Rejection is sensitive lethal doses radiation in mouse but resistant sublethal levels radiation. In an effort identify cells mediating host resistance, lymphocytes were isolated and cloned from spleens mice 7 days after TBI (650 cGy) inoculation allogeneic marrow. All clones cytolytic specificity for MHC encoded gene products donor. When transferred vivo into lethally irradiated (1025 recipients unable reject marrow, results utilizing splenic 125IUdR uptake indicated these MHC-specific cytotoxic could suppress proliferation. order characterize mechanism ability affect final engraftment, double donor chimeras constructed so 2 target populations at each other present same allograft. Results directly demonstrated CTLmore » type mediate graft characterized one products.« less