Yeast suppressor mutations and transfer RNA processing.

摘要: Publisher Summary Transfer RNA molecules are essential for protein synthesis in all organisms. Once mature-sized tRNAs produced, further interactions with enzymes or structural proteins necessary their use biosynthesis: transport from the nucleus to cytoplasm, aminoacylation by cognate aminoacyl-tRNA synthetase, binding of initiation elongation factors, and ribosomal during translation proofreading. If suppressor tRNA locus has not been cloned, loss function red pink colonies must be mapped gene analysis. This can achieved mating mutant strain appropriate tester strains, such as a containing suppressor-inactive, wild-type anticodon question. Primer-directed mutagenesis allows specific changes made within coding sequence which tested effect on expression. To determine transcription efficiency, lower temperature, shorter reaction time, template concentration slow processing reactions so that only dimeric transcript is formed, leading more accurate quantification.