Changes in cytokeratin expression accompany squamous metaplasia of the human respiratory epithelium.

摘要: To determine the characteristics of metaplastic changes nasal respiratory epithelium, distribution individual cytokeratins (CKs) was studied immunohistochemically and by two-dimensional gel electrophoresis. The authors define four types normal pseudostratified columnar epithelium: (1) transitional epithelium (first unusual CK.: no. 13); (2) stratified (increased expression CKs 4 13; 7, 8, 18 19 reduced); (3) squamous non-keratinized (appearance CK 16); (4) keratinized (expression 1 10, variable CK5 14 patterns in basal cells). These phenotypes were found simultaneously within single specimens, resulting apparent overall variability immunohistochemical staining patterns. Spatially, towards “normal” parts not abrupt but rather gradual. Biochemical data confirmed findings added 6 to pattern altered mucosa. this study suggest a stem cell metaplasia which is based on its inherent bimodal developmental programme. A gradual loss epithelial differentiation, as seen 18, paralleled appearance type CKs, e.g. 1, 10 13. Basal 5, 14, 17 maintained during process. Implications these results for general concepts process are discussed.