Detection of Human Papillomavirus in Cervical Lymph Nodes: A Highly Effective Strategy for Localizing Site of Tumor Origin

摘要: Purpose: Patients with head and neck squamous cell carcinoma (HNSCC) often come to clinical attention a mass due metastatic spread lymph nodes. Treatment is dictated by the subsequent determination of primary tumor site stage. However, remains elusive in some patients even after an exhaustive examination. Human papillomavirus type 16 (HPV-16) important etiologic agent for HNSCCs that arise within oropharynx but less so tumors from nonoropharyngeal sites. Detection HPV-16 or surrogate marker may be useful localizing origin who present HNSCC. Experimental Design: We performed situ hybridization (ISH) on node metastases 68 P16 immunohistochemistry was also because integration disrupts retinoblastoma pathway induces overexpression p16. Results: detected 22 (32%) cases ISH. When stratified origin, 31 (71%) oropharynx, none 37 (0%) other sites ( P versus 9%; 3%; Conclusions: For HNSCC, detection reliable way establish either directly ISH indirectly p16 overexpression.